Epple HJ, Amasheh S, Mankertz J, Goltz M, Schulzke JD, Fromm M (2000): Early aldosterone effect in distal colon by transcriptional regulation of ENaC subunits
Am. J. Physiol. Gastrointest. Liver Physiol. 278: G718-G724.

Aldosterone-induced sodium absorption is mediated by the epithelial Na 1 channel (ENaC). It is thought that the "early effect" is not based on genomic regulation of ENaC expression, because ENaC subunit transcription was reported to start later than Na+ transport. We investigated electrogenic Na+ absorption (J_Na) and, in identical tissues, mRNA expression of ENaC subunits in early (EDC) and late (LDC) distal colon of the rat. In both segments, 8-h in vitro incubation with 3 nM aldosterone enhanced expression of beta- and gamma-ENaC mRNAand induced J_Na. J_Na was 10 times higher in LDC than in EDC. alpha-ENaC mRNA was unchanged in EDC, whereas it decreased in LDC. In LDC, beta- and gamma-ENaC mRNA was induced 1 h after aldosterone addition, whereas J_Na became apparent .1 h later. Downregulation of alpha-ENaC mRNA did not take part in acute regulation because it started after a lag time of 3 h. Time correlation of beta- and gamma-ENaC induction and J_Na stimulation suggests that the early aldosterone effect on Na+ absorption in distal colon is caused by transcriptional upregulation of beta- and gamma-ENaC expression.