Peitz R, Weber H, Gleier K, Zimmermann S, Beutin L (2000): Nachweis von enterohämorrhagischen Escherichia coli (EHEC) in Fleischproben und Rohwürsten. Vergleich verschiedener Methoden. [Detection of enterohaemorrhagic Escherichia coli (EHEC) in raw meat and raw meat sausages - Comparative evaluation of different methods]
Fleischwirtschaft 80 (3): 71-74.

Eighty-one beef and pore meat: samples and 41 samples from raw sausages were examined for the presence of enterohaemorrhagic Escherichia coli (EHEC). Different detection and enrichment procedures for EHEC were compared. All samples were investigated by PCR methods specific for different types of shiga-toxin (stx) genes. Three (2.5 %) of the 122 samples were found positive in two different stx-specific PCRs and in the verocell toxicity assay. One of the two employed stx-PCR methods gave more specific results than the other. A liquid medium containing vancomycin, cefixime and cefsulodin which was originally developed for selective enrichment of EHEC O157 was not suitable for enrichment of non-O157 EHEC strains. Enterohaemolysin-agar containing 8mg/Lvancomycin was used as a selective medium for plating Gram-negative bacteria from meat samples. However, higher concentrations than 8 mg/L vancomycin should not be used because with these a false-positive hemolysis tvas observed with some bacteria. The O157 CD-Seiken-Test was used for specific enrichment and detection of EHEC O157 from meat samples. With this method, two colonies of EHEC O 157/10 g meat were still detectable. The test employs an immunomagnetic separation (IMS) step for concentration of E. coli O157 bacteria. However, high amounts of Eat: in the sample hindered the performance of the IMS.