Opitz B, Vinzing M, van Laak V, Schmeck B, Heine G, Slevogt H, N'Guessan PD, Goldmann T, Flieger A, Suttorp N, Hippenstiel S (2006): Legionella pneumophila induced IFNβ in lung epithelial cells via IPS-1 and IRF3 which also control bacterial replication
J. Biol. Chem. 281 (47): 36173-36179, Epub ahead of print Sep 19. doi: 10.1074/jbc.M604638200.

Legionella pneumophila, a Gram-negative facultative intracellular bacterium, causes severe pneumonia (Legionnaires´ disease). Type I IFNs were so far associated with antiviral immunity, but recent studies also indicated a role of these cytokines in immune responses against (intracellular) bacteria.

Here we show that wild-type L. pneumophila and flagellin-deficient Legionella, but not L. pneumophila lacking a functional type IV secretion system Dot/Icm, or heat-inactivated Legionella induced IFNβ expression in human lung epithelial cells. We found that IFN-regulated factor (IRF)-3 and NF-κB-p65 translocated into the nucleus and bound to the IFNβ gene enhancer after L. pneumophila infection of lung epithelial cells. RNA interference demonstrated that in addition to IRF3, the caspase recruitment domain (CARD)-containing adapter molecule interferon-β promoter stimulator 1 (IPS-1) is crucial for L. pneumophila-induced IFNβ expression, whereas other CARD-possessing molecules such as retinoic-acid-inducible protein I (RIG-I), melanoma-differentiation-associated gene 5 (MDA5), nucleotide-binding oligomerization domain protein 27 (Nod27) and apoptosis-associated speck-like protein containing a CARD (ASC) seemed not to be involved. Finally, bacterial multiplication assays in siRNA-treated cells indicated that IPS-1, IRF3 and IFNβ were essential for the control of intracellular replication of L. pneumophila in lung epithelial cells.

In conclusion, we demonstrated a critical role of IPS-1, IRF3 and IFNβ in Legionella infection of lung epithelium.