Werner G, Bartel M, Wellinghausen N, Essig A, von Baum H, Klare I, Witte W, Poppert A (2007): Detection of Resistance Mutations to Linezolid in Enterococcus spp. by Fluorescence in situ Hybridization (FISH)
J. Clin. Microbiol. 45 (10): 3421-3423. Epub May 2 [doi:10.1128/JCM.00179-07].

A fluorescence in situ hybridization (FISH) assay was established to detect linezolid resistance (mutation 2576G>T in 23S rDNA) in enterococci. The assay was evaluated on 106 Enterococcus isolates; it showed a sensitivity of 100% for the detection of phenotypic resistance and was even able to identify one single mutated allele in phenotypically linezolid-susceptible isolates.