Streker K, Schäfer T, Freiberg C, Brötz-Oesterhelt H, Hacker J, Labischinski H, Ohlsen K (2008): In vitro and in vivo validation of ligA and tarI as essential targets in Staphylococcus aureus
Antimicrob. Agents Chemother. 52 (12): 4470-4474. Epub Sep 22.

A conditional expression system has been developed using the IPTG-inducible Pspac promoter to validate essential genes of Staphylococcus aureus in vivo. The system has been applied to prove essentiality of ligA and to evaluate the essential function of tarI which was found to be essential in vitro but not in vivo.